Background There are numerous ways for blended learning and for appropriate and reasonable establishment of such methods; the use of scientific methods is essential. This study aimed at assessing the establishment of Blended Learning using the ISO 10015 in Tabriz health centers. Methods The statistical population of this descriptive study included all of the health staff of Tabriz health center during year 2016 and the sample size was set at 285, using the Morgan table. The data collection tool was a 63-item researcher-made questionnaire in five-point Likert scale based on the requirements of ISO 10015. It measured feasibility assessment, design, implementation, and evaluation of blended learning. The questionnaire’s validity was assessed by experts and its validity was calculated by Cronbach’s alpha as 0.95. Descriptive statistics and one sample t test were used to analyze the data. Results Assessment of the establishment of blended learning indicates that mean scores were calculated for needs assessment at 2.20 ± 0.34, design at 2.1 ± 0.41, implementation at 2.35 ± 0.59, and evaluation at 2.35 ± 0.48 for the implementation of blended learning, which were less than the expected mean (3) and this difference was statistically significant (P < 0.001). Conclusions These results could be used as the basis of evaluation for providing appropriate infrastructure for the establishment of blended learning.